The Q Exactive HF mass spectrometer combines a powerful quadrupole for rapid and highly accurate isolation of precursor ions with a high-resolution Orbitrap mass analyzer, enabling ultra-fast identification and quantification of precursor ions with high sensitivity. The instrument is directly coupled to an Easy-nLC 1200 chromatography system equipped with a temperature-controlled NanoFlex ion source for highly reproducible separation and ionization of analytes at the front end of the mass spectrometer.

For quantitative proteomics experiments, we primarily use data-dependent acquisition (DDA) strategies. The Q Exactive HF is suitable for the characterization of purified proteins as well as the near-comprehensive analysis of cellular proteomes. For quantification, we employ label-free, SILAC, TMT, or dimethyl labeling strategies. More information about sample preparation can be found here.

Contact: Markus Räschle, 0631-205-4269