Our group uses high-throughput genetics, microscopy and biochemistry to understand the coordination between protein translation and protein import into mitochondria. Most of mitochondrial proteins are translated on cytosolic ribosomes, complicated and ancient molecular machines. Many mitochondrial proteins are made in the cytosol, far away from mitochondria, and spend some time there before being imported. Other mitochondrial proteins are translated in close proximity to mitochondrial membrane. Why does it happen? How the fate of each protein is determined? What are the factors that regulate translation in the proximity of mitochondria? How deep does local organization of translation go next to organelle membranes and organelle subdomains? To answer these questions, we use the power of yeast genetics. In this organism, we can easily modify each gene and create custom collections of mutants that span the whole genome. Manipulating these collections in parallel we can study multiple proteins simultaneously, quantitatively compare them to each other and discover pathways that govern their import.



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