Confocal Spinning Disk Microscope:
Olympus SpinSR/ScanR (2025)
The Olympus SpinSR/ScanR is an inverted confocal spinning disk microscope equipped to allow high-throughput screening in multi-well plates aided by a robotic plate loader and providing enhanced resolution via the SoRA mode.
Microscope details
| General applications | High-throughput screening of microbial mutants in multi-well plates; z-stack imaging with confocal spinning disc; image analysis |
| Extra features | Enhanced resolution with SoRa; 3.2x magnification changer; dual cameras for simultaneous two-channel imaging; robotic plate loader |
| Microscope body | Olympus IX83 inverted motorized |
| Illumination | Yokogawa W1 spinning disk unit equipped with 405, 488, 561, and 647 nm laser lines |
| Filters | Four-color dichroic mirror for DAPI/A350, FITC/A488/GFP, TRITC/mCherry, Cy5/A647 and corresponding band pass emission filter wheels; two-camera beam splitter at 561 nm |
| Detection | 2x Hamamatsu Orca Fusion cameras |
| Objectives | Air: 10x, 20x, 40x Water: 60x NA=1.2; with water pump Oil: 60x NA=1.42, 100x NA=1.3 |
| Focus system | Infrared LED-based ZDC2 system for on demand and continuous hardware autofocus; software autofocus |
| Incubator | None |
| Software | Cellsens Dimension; ScanR Acquisition; ScanR Analysis with neural network plugins |
| Acquisition year | 2025 (funded by the EU via an ERC grant) |
| Location | Department of Cell Biology Building 13 Room 466 |
| Contact | Phone: 0631 205 2885 Email: yury.bykov(at)rptu.de |
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