Zeiss - Confocal Laser Scanning Microscope
Zeiss - Axio Observer (2025)
This confocal laser scanning microscope is equipped with a CO2-incubator and temperature control to allow for multi-colour imaging of fixed and live cells. In addition, the system offers FRAP.
Microscope details
| General applications | Multi-colour imaging of fixed and live mammalian cells; widefield, Apotome-based optical sectioning and confocal laser scanning microscopy (e.g. FRAP, z-stacks, time-lapse) |
| Extra features | Apotome 3 for confocal-like LED imaging; multiband and single bandpass filters for high image quality and minimal bleed-through |
| Microscope body | Axio Observer (Zeiss), inverted research microscope |
| Illumination | Viluma 7 LED light source for widefield imaging; LSM 900 laser scanning confocal illumination |
| Filter cubes | Multiband and single bandpass filter sets available for most LED channels |
| Detection | Axiocam 702 monochrome camera (widefield); spectral photomultiplier tube (PMT) detectors with multialkali photocathodes (LSM 900) |
| Objectives | Zeiss Plan-Apochromat 20×; Plan-Apochromat 63× oil; LCI Plan-Neofluar 63× water |
| Focus system | Definite Focus 2 hardware-based focus stabilization |
| Incubator | Environmental incubation chamber with temperature and CO₂ control |
| Software | ZEN (Zeiss) imaging software |
| Acquisition year | 2025 (upgrade to Colibri 7 LED illumination and LSM 900 confocal module) |
| Location | Human Biology and Human Genetics Building 13 Room 103 |
| Contact | Stefan Kins, 0631 205 2106 |
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