Epifluorescence Microscope
Zeiss - High Speed Cell Observer with ApoTome (Axio Observer Z.1; 2009)
The High Speed Cell Observer microscope is an inverted epifluorescence microscope with ApoTome module for structured illumination to obtain optical sections. The microscope is equipped for live cell imaging with a temperature-controlled incubator and a CO2 module. It can be used for routine imaging of fixed and living cells.
Microscope details
| General applications | Multi-colour imaging of fixed and live mammalian cells based on dyes such as DAPI (405 nm) and Alexa (488, 568, 594, 647 nm) Interaction studies |
| Extra features | ApoTome, time lapse imaging, 3D display, colocalization, Z-stack, tiles + positions (widefield) => creation of images that are larger than the actual image section |
| Microscope body | Inverted |
| Illumination | LED light source Colibri HXP (fluorescence) Halogen (transmitted light) |
| Filter cubes | GFP (green) = set38 (BP 470/40, FT 495, BP 525/50); PI (red): set00 (BP 530-585, FT 600, LP 615) |
| Detection | Axiocam 702 Novo |
| Objectives | 20x, 40x, 63x (oil), 63x (glycerol), 100x (oil) |
| Focus system | Definite Focus autofocus system |
| Incubator | Yes, temperature-control, CO2-module |
| Software | Zen blue (Zeiss) |
| Acquisition year | 2009 |
| Location | Human Biology and Human Genetics Building 13 Room 103 |
| Contact | Stefan Kins, 0631 205 2106 |
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