Confocal Laser Scanning & STED Microscope
Leica Stellaris 8 tau-STED FALCON
The Leica Stellaris 8 is a high end confocal microscope equipped with a 775 nm laser for STED super resolution microscopy. Since it does not have a CO2 incubator, it is best equipped for the confocal and super resolution imaging of fixed samples and live non mammalian cells such as from Drosophila melanogaster.
Microscope details
| General applications | Confocal imaging of fixed and live samples (S1). Super-resolution STED imaging using tau-STED and FALCON module. Live-time imaging. Multi-color imaging |
| Extra features | STED, 3D-STED, FALCON, fast live-imaging (8kHz tandem Scanner), FLIM |
| Microscope body | inverted DMi8 |
| Illumination | White light laser WLL: 440-790nm 488 nm laser for FRAP 775 nm STED laser |
| Filter cubes | FI/TRITC, GFP, TXR, illumination via LED Acousto-Optical-Tunable-Filter AOTF: emission wavelength in 1nm-steps within 440-790nm spectra free selectable |
| Detection | Spectral detectors 2 HyD S and 2 hybrid detectors HyD X and a HyD R detector. Detection range: 410-850 nm Transmission light detection |
| Objectives | HCPL FLUOTAR 10x/0.30 |
| Focus system | closed loop focus |
| Incubator | Incubator for temperature control |
| Software | LAS X |
| Acquisition year | 2021 (funded by DFG INST 248/293-1 FUGG) |
| Location | Department of Zoology Imaging Facility, Building 13, Room 155.3 |
| Contact | Prof. Dr. Jan Pielage, 0631 205 2426 |
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