PDFs of the lectureMass Spectrometry and Deep Sequencing based Strategies in Systems Biology

I. Definitions of "Systems Biology"

II. "Deep Sequencing" or "Next Generation Sequencing" technologies

III. Fundamentals of mass spectrometry for protein analysis

IV. Peptide identification by mass spectrometry

V. Quantitative proteome analysis

VI. data-dependent (DDA) versus data-independent acquisition (DIA)

Literature to read

NGS: Goodwin et al, 2016

MS: Mann and Aebersold, 2016

MS: Wienkoop et al, 2010

MS: Hammel et al, 2018

Questions and Solutions

Solution to de novo sequencing question

Animations

Linear Ion trap by Thermo Scientific

Orbitrap Elite

SWATH by ABSciex

Literature

Textbook

Example exam

WS 2017/18