Downloads of the lecture BIO-BTE-02-V-2

PDFs of the lecture

General information

I. Introduction and definition of biotechnology

II. basics of prokaryotic and eukaryotic gene expression

III Recombinant DNA technology

IV. Chemical synthesis, amplification and sequencing of DNA

V. Alternative cloning methods and synthetic biology

VI Quantification of nucleic acids

VII Expression of foreign proteins in bacteria

VIII Expression of foreign proteins in eukaryotes

IX. Directed mutagenesis and tailoring of proteins

Link to video recordings of the lectures

 

Slides VL PD Dr. Erkel

Fermentation 1

Fermentation 2

 

Videos from the lecture (> 10 MB as link)

Video 1 - Transcription

Video 2 - Splicing

Video 3 - Translation

Video 4 - Lac operon

Video 5 - trp operon

Video 6 - Initiation at the eukaryotic promoter

Video 7 - Packaging of DNA in eukaryotes

Video 8 - Gene silencing by DNA methylation

Video 9 - Protein secretion

Video 10 - Cloning with E.coli

Video 11 - Agarose gel electrophoresis

Video 12 - EcoRI and ligase

Video 13 - Lambda phage

Video 14 - PCR

Video 15 - DNA sequencing according to Sanger

Video 16 - Reading the sequence with fluorescent dyes

Video 17 - Gibson Assembly

Video 18 - Golden Gate Cloning

Video 19 - Microarray spotting robot

Video 20 - Photolithographic probe synthesis

Video 21 - Array Gene Expression

Video 22 - Real-time PCR

Video 23 - Baculovirus life cycle

 

Exams of the last years

Exam 2012 major subject, with answers

Exam 2012 minor subject/teaching profession, with answers

Post-exam 2012 major subject, with answers

Post-exam 2012 minor subject/teaching profession, with answers

Exam 2013 major subject, with answers

Exam 2013 minor subject/teaching profession, with answers

Post-exam 2013 major subject, with answers

Post-exam 2013 minor subject/teaching profession, with answers

Exam 2014 major subject, with answers

Exam 2014 minor subject/teaching profession, with answers

Exam 2015 major subject, with answers

Exam 2015 minor subject/teaching profession, with answers

Post-exam 2015 major subject, with answers

Post-exam 2015 minor subject/teaching profession, with answers

Exam 2016 major subject, with answers

Exam 2016 minor subject/teaching profession, with answers

Post-exam 2016 major subject, with answers

Post-exam 2016 minor subject/teaching profession, with answers

Exam 2017 major subject, with answers

Exam 2017 minor subject, with answers

 

 

Appointment to discuss the old exams:

Will take place on Tuesday, Sept. 18 from 10-12 in 13-305.

 

Exam dates

Main exam: Wednesday 26.9.2018, 8.30 to 11.00, Mensa (start of exam: 8.30).

Post-exam: Monday 22.10.2018, 8.30 to 11.00, Mensa (start of exam: 8.30).

(due to the current situation: compulsory registration in QIS; exams of non-registered students will not be corrected!)