Maintenance of Genomic Stability


Dr. Markus Räschle
Molecular Genetics
TU Kaiserslautern
Paul-Ehrlich-Straße 24, Room 138B
67663 Kaiserslautern

Tel.: +49 (0)631-205-4269
Fax: +49 (0)631-205-4090

Web:   Molecular Genetics
Email:  raeschle[at]
Profile: Google Scholar

Curriculum Vitae:

since 2016: Project Leader in the Group of Professor Storchova at the Departmentof Biology, University Kaiserslautern, Germany.

2011-2016: Research Fellow with Professor Matthias Mann, Max-Plack Institute of Biochemistry, Martinsried, Germany.

2009-2011: Research Fellow with Professor Stefan Jentsch, Max-Plack Institute of Biochemistry, Martinsried, Germany.

2004-2008: Postdoctoral Researcher with Professor Johannes Walter, Harvard Medical School, Boston, USA.

2001-2004: Postdoctoral Researcher with Professor Thomas Ellenberger, Harvard Medical School, Boston, USA.

1996-2001: Ph.D. Studies in the Laboratory of Professor Josef Jiricny, Institute of Medical Radiobiology, Swiss Federal Institute of Technology (ETH), Switzerland.

Major Research Interests:

Our group uses quantitative mass spectrometry to monitor global proteome changes upon various perturbations. One major research focus is DNA repair. Using Xenopus egg extracts we study how DNA lesions are repaired in the context of ongoing DNA replication. We develop mass spectrometry workflows to monitor protein recruitment and damage signaling during the repair process. In biochemical assays we dissect the molecular events required to bypass DNA lesions and to maintain genomic stability during cell division.  research pages

Selected Publications:

The intermembrane space protein Mix23 is a novel stress-induced mitochondrial import factor.
Zöller E, Laborenz J, Krämer L, Boos F,Räschle M, Alexander RT, Herrmann JM. J Biol Chem (2020):
295(43):14686-14697. doi: 10.1074/jbc.RA120.014247. Epub 2020 Aug 21.

The CMG helicases bypasses DNA-protein crosslinks to facilitate their repair.
Sparks JL, Chistol G, Gao AO, Räschle M, Larsen NB, Mann M, Duxin JP and JC. Walter. Cell (2019):176(1-2):167-181.

Duxin.Replication-coupled DNA –protein crosslink repair by SPARTN and the proteasome in Xenopus egg extracts.
Larsen NB, Gao AO, Sparks JL, Gallina I, Wu RA, Mann M, Räschle M, Walter JC and JP. Duxin. Mol Cell (2019): 73(3):574-588

CRL2Lrr1 promotes unloading of the vertebrate replisome from chromatin during replication termination.
Dewar, J. M., Low, E., Mann, M., Räschle, M*., and J.C. Walter* Genes Dev. (2017):31:275-290.

Activation of the ATR kinase by the RPA-binding protein ETAA1.
Haahr, P., Hoffmann, S., Tollenaere, M.A., Ho, T., Toledo, L.I., Mann, M., Bekker-Jensen, S., Räschle, M*., and N. Mailand*. Nature Cell Biol. (2016):18:1196-1207.

SCAI promotes DNA double-strand break repair in distinct chromosomal contexts.
Hansen, R.K., Mund, A., Poulsen, S.L., Sandoval, M., Klement, K., Tsouroula, K., Tollenaere, M.A., Räschle, M., Soria, R., Offermanns, S., Worzfeld T, Grosse R, Brandt DT, Rozell B, Mann M, Cole F, Soutoglou E, Goodarzi AA, Daniel JA, Mailand N and S. Bekker-Jensen Nature Cell Biol. (2016):18, 1357-1366.

TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress.
Hoffmann, S., Smedegaard, S., Nakamura, K., Mortuza, G.B., Räschle, M., Ibanez de Opakua, A., Oka, Y., Feng, Y., Blanco, F.J., Mann, M., Montoya G, Groth A, Bekker-Jensen S and N. Mailand. J Cell Biol. (2016):212, 63-75.

Homology-driven assembly of NOn-redundant protEin sequence sets (NOmESS) for mass spectrometry.

Temu, T., Mann, M., Räschle, M.*, and Cox, J.* Bioinformatics. (2016):32:1417-1419.

Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links.
Räschle, M., Smeenk, G., Hansen, R.K., Temu, T., Oka, Y., Hein, M.Y., Nagaraj, N., Long, D.T., Walter, J.C., Hofmann, K., et al. 2015. DNA repair. Science. (2015):348:1253671.

Mechanism of RAD51-dependent DNA interstrand cross-link repair.
Long, D.T., Räschle, M., Joukov, V., and J.C. Walter. 2011. Science (2011): 333:84-87.

The Fanconi anemia pathway promotes replication-dependent DNA interstrand cross-link repair. Knipscheer, P., Räschle, M., Smogorzewska, A., Enoiu, M., Ho, T.V., Scharer, O.D., Elledge, S.J., and J.C. Walter. Science (2009): 326:1698-1701.

Mechanism of replication-coupled DNA interstrand crosslink repair.
Räschle, M., Knipscheer, P., Enoiu, M., Angelov, T., Sun, J., Griffith, J.D., Ellenberger, T.E., Scharer, O.D., and J.C. Walter. Cell (2008): 134:969-980.

* indicates joint correspondence

Complete list of publications