Confocal Laser Scanning-microscope with FCS and FLIM-modul

Leica Stellaris 8 FALCON + FCS (2023)

Stellaris 8 is a true confocal point scanning system, including a White Light Laser as excitation light source, an Acousto Optical Beam Splitter (AOBS) and a highly sensitive, prism-based spectral detection design with computer controlled adjustable bandwidth for all fluorescence channels. The FALCON module  includes FLIM analysis tools, such as exponential fitting, FLIM phasor analysis, pattern fitting and FLIM-FRET analyzer. It allows to sense physical and chemical properties and to quantify fast processes, like molecular interaction or molecular environment changes. The FCS module system provides a solution for fluorescence correlation and crosscorrelation spectroscopy (FCS/FCCS) measurements. It allows to measure local flow velocities and migration velocieties of molecules as well as diffusion times, gives insight into dynamics of the energy states of a fluorophore like triplet or blinking dynamics and much more.

Microscope details

Microscopy of biological and technical microsystems. Dynamics very close to interfaces and in fluid boundary layers. Sensing of physical and chemcial properties via fluorescence lifetime. Measurement of local flow velocities as well as single particle migration, diffusion, substance concentrations, reaction rates, pH, triplet/blinking. Local viscosities, protonation, size/hydrodynamic radius of (single-)molecules and aggregate formation dynamics.
Fluorescence Correlation Spectroscopy FCS (and Cross Correlation FCCS),
Fluorescence Lifetime Imaging FLIM (and lifetime unmixing/decay analysis),
Fluorescence Lifetime Correlation Spectroscopy FLCS and
Förster Resonance Energy Transfer FRET. FLIM-FRET



IlluminationWhite light laser WLL: 440-790nm
Filter cubesAcousto-Optical-Tunable-Filter AOTF: 
emission wavelenght in 1nm-steps within 440-790nm spectra free selectable
DetectionSpectral detector HyD S and 2 hybrid detectors HyD X detection range: 410-850 nm 
ObjectivesHC PL APO 63x/1.20 W HCPL FLUOTAR 10x/0.30 dry
Focus systemManually/ autofocus
SoftwareLAS X FCS and FLIM
2023 (funded by DfG)

Institute of Microfluidmechanics, 52/ 219
probably at the end of the year 2024 in 56/ 263,


Jan-Niklas Schäfer,
0631 205 4424

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