Epifluorescence Microscope
Leica – THUNDER Imager 3D Live Cell (2021)
Leica's THUNDER Imager 3D Live Cell is an inverted epifluorescence microscope for multi-colour imaging of live and fixed cells. It is equipped with an incubator including temperature- and CO2-control modules. Based on the use of computational clearing this microscope can also be used to resolve details in thick samples in real time.
Microscope details
| General applications | Multi-colour imaging of fixed and live cells, especially used for yeast cells |
| Extra features | Computational clearing for imaging thick samples |
| Microscope body | DMI8 inverted Fully motorised |
| Illumination | High speed LED8 with high speed emission filter wheel (8 LEDs: 395, 438, 475, 511, 555, 575, 635, 730 nm |
| Filter cubes | LED8 emission filter wheel (460/80; 535/70; 590/50; 642/80); Filter cube CYR71010 (excitation: 436/28, 506/21, 578/24, 730/40; Dichroic mirror: 459, 523, 598, 763; emission: 473/22, 539/24, 641/78, 810/80); Filter cube DFT51010 (excitation: 391/32, 479/33, 554/24, 638/31; Dichroic mirror: 415, 500, 572, 660; emission: 435/30, 519/25, 594/32, 695/58) |
| Detection | Leica DFC9000 GTC (4.2 MP monochrome sCMOS camera with 82% max QE; 6.5 µm pixel size) |
| Objectives | N PLAN 5x/0.12 PH0 HC PL FLUOTAR 10x/0.32 PH1 HC PL FLUOTAR L 40x/0.60 CORR PH 2 HC PL APO 40x/1.30 Oil HC PL APO 100x/1.44 Oil CORR CS1150 |
| Focus system | Hardware autofocus Leica AFC including closed loop focus |
| Incubator | Incubator i8 with temperature- and CO2-control |
| Software | LAS X (Live Data Mode, Trigger to peripherals, 3D Analysis, 3D Visualization Advanced) |
| Acquisition year | 2021 (DFG, INST 248/336-1) |
| Location | Department of Cell Biology |
| Contact | Katja Hansen, |
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