Confocal Spinning Disc Microscope (with FRAP, TIRF, SR)
Nicon Confocal CSU-W1 SORA (2021)
This confocal spinning disk microscope is equipped with a CO2-incubator, temperature control and a perfect focus system to allow not only for high temporal resolution, but also for undisturbed imaging over extended time intervals. In the SORA modus the microscope provides super resolution imaging via optical photon reassignment. In addition, the system offers TIRF microscopy and FRAP.
Microscope details
| General applications | Microscopy of fixed and live mammalian cells based on dyes like Dapi (405) & Alexa (488, 568, 647); fast as well as long-lasting live cell imaging with stable focus (also in super resolution mode (~100nm)); FRAP, TIRF; automated imaging |
| Extra features | DIC, FRAP, TIRF, super resolution modus SORA |
| Microscope body | Ti2-E inverted, motorized |
| Illumination | Laser (405, 488, 561, 638 nm); TI2-D-LHLED |
| Filter cubes | DAPI/A350; FITC/A488; TRITC/A546; Cy5/A647 |
| Detection | 2 sCMOS cameras; MXR10015 |
| Objectives | 20x, 40x, 60x, 100x (TIRF) |
| Focus system | Perfect Focus System PFS4 (offset lense) |
| Incubator | Yes, CO2 and temperature control |
| Software | NIS Elements AR with various plug-ins |
| Acquisition year | 2021 (funded by DFG INST 248/342-1, project number 461336323) |
| Location | Department of Nanophysiology Building 13, Room 155.3 |
| Contact | Tanja Maritzen, 0631 205 4908 |
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