01 Zeiss LSM880 Microbiology - Fachbereich Biologie an der RPTU

Confocal Laser Scanning Microscope

Zeiss - LSM 880 (2017)

The LSM 880 is an inverted confocal laser scanning microscope. For live cell imaging the microscope is equipped with a temperature-controlled incubator and a CO₂ module.

Image of Zeiss LSM880 microscope — LSM 880 Setup

Zeiss LSM880 microscope Image of roots — Vacuoles in cortex cells in the root tip of Arabidopsis thaliana were stained with the fluorescent pH indicator BCECF and with the vacuole reporter spRFP-AFVY (courtesy of David Scheuring).

Microscope details

General applications	High resolution multi-colour imaging of fixed cells Fast high resolution multi-colour imaging of living cells
Extra features	Time lapse Multiwell FRAP Located in S2 room
Microscope body	Inverted Axio Observer
Illumination	Laser (405, 488, 514, 543, 594, 633 nm) LED light source Colibri
Filter cubes
Detection	Quasar detector (spectral detector, GaAsP PMT) for high sensitivity Transmitted light PMT
Objectives	Plan-Apochromat 5x/0.16 Plan-Apochromat 20x/0.8 C Apo 40x/1.2 W Long Distance (LD) LCI Plan-Apochromat 40x/1.2 W Pln Apo 63x1.4 oil
Focus system
Incubator	Yes, temperature-control, CO₂-module
Software	Imaris for data analysis available
Acquisition year	2017 (funded by DFG: INST 248/254-1)
Location	Department of Microbiology Building 23, Room 161
Contact	Susanne Zehner, 0631 205 2503

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