564. WE-Heraeus-Seminar: Physical Approaches to Membrane Proteins
25th−28th May 2014
Physikzentrum Bad Honnef, Germany
Scientific Programme (pdf)
| Sunday, 25th May | |
|---|---|
| 16:00 | Arrival, registration, setting up posters, coffee |
| 18:30 | Reception |
| 20:00 | Welcome remarks Sandro Keller, Molecular Biophysics, University of Kaiserslautern, Germany |
| Segmented folding of a bacterial membrane protein Daniel E. Otzen, iNANO, Department of Molecular Biology and Genetics, Aarhus University, Denmark | |
| Monday, 26th May | |
| 07:30 | Breakfast |
| Session 1: Emerging concepts in membrane-protein folding Chair: Sebastian Fiedler, Leslie Dan Faculty of Pharmacy, University of Toronto, Canada | |
| 08:30 | Introduction |
| 08:45 | Folding α-helical membrane proteins Paula J. Booth, School of Biochemistry, University of Bristol, UK |
| 09:30 | Folding and activity of the tetrameric E. coli glycerol facilitator GlpF in lipid bilayers and membrane-mimetic environments Dirk Schneider, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany |
| 10:00 | Poster flashes |
| 10:30 | Poster session/coffee |
| 11:15 | The versatile β-barrel gives up secrets of the membrane Karen G. Fleming, Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, USA |
| 12:00 | Assisted insertion and folding of outer membrane proteins from Escherichia coli into lipid membranes Jörg H. Kleinschmidt, Department of Biophysics, University of Kassel, Germany |
| 12:30 | New insights into the molecular mechanism of β-barrel outer membrane protein folding David J. Brockwell, School of Molecular and Cellular Biology, University of Leeds, UK |
| 13:00 | Lunch |
| Session 2: Model systems of membranes and membrane proteins Chair: Katharina Gimpl, Molecular Biophysics, University of Kaiserslautern, Germany | |
| 14:30 | Introduction |
| 14:45 | Solubilisation of membranes into nanodiscs by amphipathic polymers J. Antoinette Killian, Membrane Biochemistry and Biophysics, Utrecht University, The Netherlands |
| 15:30 | Parameters governing the activity, selectivity, and concerted action of membrane-active peptides and (bio)surfactants Heiko Heerklotz, Leslie Dan Faculty of Pharmacy, University of Toronto, Canada |
| 16:00 | Poster flashes |
| 16:30 | Poster session/coffee |
| 17:15 | Pore-spanning membranes as a tool to monitor protein-mediated fusion processes Claudia Steinem, Institute of Organic and Biomolecular Chemistry, Georg August University, Göttingen, Germany |
| 18:00 | Characterising in vitro membrane-assisted protein synthesis (iMAPS) and co-translational insertion into polymer membranes Eva-Kathrin Sinner, Institute of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria |
| 18:30 | Non-ionic amphiphilic polymers and fluorinated surfactants: two convenient alternatives to detergents for the study of membrane proteins Grégory Durand, Equipe Chimie Bioorganique et Système Amphiphiles, Université d'Avignon et des Pays de Vaucluse, Avignon, France |
| 19:00 | Dinner |
| 20:30 | Transporters of the multidrug resistance: reconstitution into proteoliposomes and biophysical characterisation Hans-Joachim Galla, Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Germany |
| Tuesday, 27th May | |
| 07:30 | Breakfast |
| Session 3: Computational approaches to membrane proteins Chair: Georg Krainer, Molecular Biophysics, University of Kaiserslautern, Germany | |
| 08:30 | Introduction |
| 08:45 | Membrane proteins in context: molecular simulations of membrane proteins and their lipid interactions Mark S. P. Sansom, Department of Biochemistry, University of Oxford, UK |
| 09:30 | Hydrogen bonding and lipid interactions in membrane protein function Ana-Nicoleta Bondar, Department of Physics, Freie Universität Berlin, Germany |
| 10:00 | Poster flashes |
| 10:30 | Poster session/coffee |
| 11:15 | Mechanoenzymatics: forces and conformational dynamics in biomolecular nanomachines Helmut Grubmüller, Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany |
| 12:00 | Multi-scale simulations of bacterial membranes: successes and outlook for the future Syma Khalid, Systems and Synthetic Biology Modelling Group, University of Southampton, UK |
| 12:30 | The mechanism of channel protein gating, solute selectivity, and flux Martin B. Ulmschneider, Department Materials Science and Engineering, Johns Hopkins University, Baltimore, USA |
| 13:00 | Lunch |
| Session 4: New and notable Chair: Johannes Klingler, Molecular Biophysics, University of Kaiserslautern, Germany | |
| 14:30 | Introduction |
| 14:45 | Structure, dynamics, and function of Opa60: a Neisserial ß-barrel membrane protein that mediates host phagocytosis Linda Columbus, Department of Biochemistry, University of Virginia, Charlottesville, USA |
| 15:15 | Infrared spectroscopy on lipid–protein interactions: what crystals don't tell Karim Fahmy, Biophysics Division, Helmholtz Centre Dresden-Rossendorf, Germany |
| 15:45 | Optimising SDS-PAGE for membrane proteins Arianna Rath, Division of Molecular Structure and Function, Hospital for Sick Children, Toronto, Canada |
| 16:30 | Excursion |
| 19:00 | Conference dinner |
| 20:30 | The role of the membrane in protein pattern formation Petra Schwille, Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany |
| Wednesday, 28th May | |
| 07:30 | Breakfast |
| Session 5: Methodological advances in the study of membrane proteins Chair: Carolyn Vargas, Molecular Biophysics, University of Kaiserslautern, Germany | |
| 08:30 | Introduction |
| 08:45 | Surface-enhanced IR spectroscopy probes membrane protein folding and function Joachim Heberle, Department of Experimental Molecular Biophysics, Freie Universität Berlin, Germany |
| 09:30 | Hydrodynamic and scattering characterisation of membrane proteins and surfactants Christine Ebel, Institut de Biologie Structurale, Grenoble, France |
| 10:00 | Poster flashes |
| 10:30 | Poster session/coffee |
| 11:15 | Investigating membrane protein folding James U. Bowie, Department of Chemistry and Biochemistry, University of California, Los Angeles, USA |
| 12:00 | GPCR-cholesterol interaction: a multidimensional approach Amitabha Chattopadhyay, Centre for Cellular and Molecular Biology, Hyderabad, India |
| 12:30 | Transport machineries in biomembranes utilising electrostatic charge zippers Anne S. Ulrich, Institute of Biological Interfaces 2, Karlsruhe Institute of Technology, Germany |
| 13:00 | Poster award/lunch |
| 14:30 | Departure |