Fachgebiet Molekulare Biophysik

564. WE-Heraeus-Seminar: Physical Approaches to Membrane Proteins

25th−28th May 2014
Physikzentrum Bad Honnef, Germany

Scientific Programme (pdf)

Sunday, 25th May
16:00Arrival, registration, setting up posters, coffee
20:00Welcome remarks
Sandro Keller, Molecular Biophysics, University of Kaiserslautern, Germany
Segmented folding of a bacterial membrane protein
Daniel E. Otzen, iNANO, Department of Molecular Biology and Genetics, Aarhus University, Denmark
Monday, 26th May
Session 1: Emerging concepts in membrane-protein folding
Chair: Sebastian Fiedler, Leslie Dan Faculty of Pharmacy, University of Toronto, Canada
08:45Folding α-helical membrane proteins
Paula J. Booth, School of Biochemistry, University of Bristol, UK
09:30Folding and activity of the tetrameric E. coli glycerol facilitator GlpF in lipid bilayers and membrane-mimetic environments
Dirk Schneider, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany
10:00Poster flashes
10:30Poster session/coffee
11:15The versatile β-barrel gives up secrets of the membrane
Karen G. Fleming, Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, USA
12:00Assisted insertion and folding of outer membrane proteins from Escherichia coli into lipid membranes
Jörg H. Kleinschmidt, Department of Biophysics, University of Kassel, Germany
12:30New insights into the molecular mechanism of β-barrel outer membrane protein folding
David J. Brockwell, School of Molecular and Cellular Biology, University of Leeds, UK
Session 2: Model systems of membranes and membrane proteins
Chair: Katharina Gimpl, Molecular Biophysics, University of Kaiserslautern, Germany
14:45Solubilisation of membranes into nanodiscs by amphipathic polymers
J. Antoinette Killian, Membrane Biochemistry and Biophysics, Utrecht University, The Netherlands
15:30Parameters governing the activity, selectivity, and concerted action of membrane-active peptides and (bio)surfactants
Heiko Heerklotz, Leslie Dan Faculty of Pharmacy, University of Toronto, Canada
16:00Poster flashes
16:30Poster session/coffee
17:15Pore-spanning membranes as a tool to monitor protein-mediated fusion processes
Claudia Steinem, Institute of Organic and Biomolecular Chemistry, Georg August University, Göttingen, Germany
18:00Characterising in vitro membrane-assisted protein synthesis (iMAPS) and co-translational insertion into polymer membranes
Eva-Kathrin Sinner, Institute of Nanobiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria
18:30Non-ionic amphiphilic polymers and fluorinated surfactants: two convenient alternatives to detergents for the study of membrane proteins
Grégory Durand, Equipe Chimie Bioorganique et Système Amphiphiles, Université d'Avignon et des Pays de Vaucluse, Avignon, France
20:30Transporters of the multidrug resistance: reconstitution into proteoliposomes and biophysical characterisation
Hans-Joachim Galla, Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Germany
Tuesday, 27th May
Session 3: Computational approaches to membrane proteins
Chair: Georg Krainer, Molecular Biophysics, University of Kaiserslautern, Germany
08:45Membrane proteins in context: molecular simulations of membrane proteins and their lipid interactions
Mark S. P. Sansom, Department of Biochemistry, University of Oxford, UK
09:30Hydrogen bonding and lipid interactions in membrane protein function
Ana-Nicoleta Bondar, Department of Physics, Freie Universität Berlin, Germany
10:00Poster flashes
10:30Poster session/coffee
11:15Mechanoenzymatics: forces and conformational dynamics in biomolecular nanomachines
Helmut Grubmüller, Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
12:00Multi-scale simulations of bacterial membranes: successes and outlook for the future
Syma Khalid, Systems and Synthetic Biology Modelling Group, University of Southampton, UK
12:30The mechanism of channel protein gating, solute selectivity, and flux
Martin B. Ulmschneider, Department Materials Science and Engineering, Johns Hopkins University, Baltimore, USA
Session 4: New and notable
Chair: Johannes Klingler, Molecular Biophysics, University of Kaiserslautern, Germany
14:45Structure, dynamics, and function of Opa60: a Neisserial ß-barrel membrane protein that mediates host phagocytosis
Linda Columbus, Department of Biochemistry, University of Virginia, Charlottesville, USA
15:15Infrared spectroscopy on lipid–protein interactions: what crystals don't tell
Karim Fahmy, Biophysics Division, Helmholtz Centre Dresden-Rossendorf, Germany
15:45Optimising SDS-PAGE for membrane proteins
Arianna Rath, Division of Molecular Structure and Function, Hospital for Sick Children, Toronto, Canada
19:00Conference dinner
20:30The role of the membrane in protein pattern formation
Petra Schwille, Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Martinsried, Germany
Wednesday, 28th May
Session 5: Methodological advances in the study of membrane proteins
Chair: Carolyn Vargas, Molecular Biophysics, University of Kaiserslautern, Germany
08:45Surface-enhanced IR spectroscopy probes membrane protein folding and function
Joachim Heberle, Department of Experimental Molecular Biophysics, Freie Universität Berlin, Germany
09:30Hydrodynamic and scattering characterisation of membrane proteins and surfactants
Christine Ebel, Institut de Biologie Structurale, Grenoble, France
10:00Poster flashes
10:30Poster session/coffee
11:15Investigating membrane protein folding
James U. Bowie, Department of Chemistry and Biochemistry, University of California, Los Angeles, USA
12:00GPCR-cholesterol interaction: a multidimensional approach
Amitabha Chattopadhyay, Centre for Cellular and Molecular Biology, Hyderabad, India
12:30Transport machineries in biomembranes utilising electrostatic charge zippers
Anne S. Ulrich, Institute of Biological Interfaces 2, Karlsruhe Institute of Technology, Germany
13:00Poster award/lunch